Microfluidic deep immune phenotyping (μDIP)

We developed an innovative living single-cell surface-anchored technology for multi-dimensional immune functional analysis, enabling the simultaneous measurement of secretions, surface proteins, and cytotoxic capacity for microfluidic deep immune profiling (μDIP). This technology provides high flexibility for detecting diverse secretions through antibody or aptamer binding and labeling, along with high-throughput screening and sorting capabilities. Furthermore, it allows real-time monitoring of cell communication by tracking biomolecular dynamics in vivo, offering deeper insights into immune cell interactions and functionality.

Intelligent microfluidic cancer cell sorter (IMC)

We developed a novel intelligent microfluidic cancer cell sorter (IMC) for advanced single-cancer-cell incubation within droplets, enabling functional testing. After extended incubation, cancer cells are processed through our AI-powered intelligent cell sorter, which efficiently isolates rare, resistant cells based on their evolutionary trajectories. Transcriptomic analysis then provides a molecular characterization of these cells, offering novel insights into intratumor heterogeneity (ITH) and facilitating the identification of treatment-predictive biomarkers for precision medicine.

Single-cell molecular sensors (SMS) for bio-fabrication

Synthetic biology modifies microbial metabolic pathways to create cell factories for producing valuable chemicals. Using a design-build-test cycle, a large yeast mutant library is screened for desired secretory traits, driving directed evolution for biofabrication. Current single-yeast secretion analysis lacks sensitivity, throughput, and speed for large-scale metabolic screening (>10^7 cells). To overcome this, we are developing a microfluidic based single-cell molecular sensor (SMS) for ultra high-speed, high-throughput single-cell secretion screening (~10^4 cells/s). This technology has identified rare strains producing valuable chemicals, advancing bio-fabrication.

Cell mimetic capsules (CMC)

Living cells execute logical cascade reactions to regulate physiological responses in a timely manner. We present a microfluidic assembly technology for fabricating cell-mimetic capsules (CMC) capable of performing cascade chemical reactions for on-demand drug delivery. Notably, by leveraging interactions between tannic acid (TA) and polyethylene glycol (PEG), we create a novel fluidic membrane surrounding the capsules. This membrane ensures both structural stability and molecular fluidity, enabling efficient transport and logic-based catalysis. Additionally, it supports flexible post-functionalization, facilitating spontaneous nanomaterial assembly. This technology advances cell-mimetic biochemical reactions, offering new possibilities for targeted drug delivery and therapeutic applications.