Research  ■

Deep immune phenotyping (DIP)

Immunotherapy, particularly adoptive cell therapy, harnesses a patient’s immune system to usher in a new era of personalized medicine, offering hope for curative treatments in severe illnesses. Despite its success, patient responses vary widely, with some experiencing nonhealing effects or unpredictable toxicity. These variations may arise from cell heterogeneity and interpatient differences in the infused cell product, highlighting the need for a comprehensive, multidimensional characterization of single cells at a systems level—yet no platform currently achieves this at scale. In this project, we developed a novel microfluidic droplet barcoding technology for rapid deep immune phenotyping (DIP), enabling high-throughput, multiplexed analysis of single-cell phenotype, including cytokine secretion and cytotoxicity. By uncovering DIP, tumor-specific immune cells can be rapidly detected and isolated, enabling effective precision immunotherapy.

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